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TEM examinations of the cysts of the control group failed to reveal endosymbionts.  We believe that this is the first report of a bacterial symbiont in Giardia lamblia. Introduction Giardia, perhaps the earliest described intestinal protozoan, has recently been the subject of renewed research interest with the recognition of (a) its pathogenicity and (b) its involvement in epidemics of waterborne diarrheal disease. One area of interest has been the study of the ultrastructure of these protozoa; it is to be hoped that an understanding of the cell biology of Giardia may include an understanding of an effective defense strategy against these parasites.

Some trophozoites were swimming freely in the medium and showed active movement and division. Figure 1.  Giemsa × 400.  At the end of the interaction period (96 h) the cell monolayer was replaced completely by the parasite monolayer.  ND1, ND2, ND3 and Portland 1 on interacted HeLa cells.  These changes consisted of retraction of cytoplasm in some cells (6 h, Figure 2B) loss of cell to cell contact with slight vacuolation of cytoplasm (24 h, Page 35 Figure 2.  48 h fibroblast cells showing loss of intercellular contacts, multiple nuclei and abnormal cytoplasm.

Isoenzyme studies of Giardia lamblia isolated from symptomatic cases.  64:119­126.  54:1047­1056.  Plenum, New York.  56:849­882.  70:443­445.  86:1488­1495.  39:101­105.  17:1­47.  Path.  39:163­170.  Sci. 26:700­704.  36:295­307.  74:213­215.  298:319­321. Page 39 Studies on the Prevalence of Giardiasis in Czechoslovakia Michal Giboda Institute of Parasitology, Czechoslovak Academy of Sciences, 370 05 Ceske Budejovice Branišovska 31, Czechoslovakia.  The author suggests that the theory of familial occurrence of giardiasis is valid universally.

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