By Karen S. Canady (auth.), Shirley Pease, Thomas L. Saunders (eds.)
This laboratory handbook, released in cooperation with the overseas Society for Transgenic expertise (ISTT), offers just about all present tools that may be utilized to the construction and research of genetically transformed animals. The chapters were contributed through top scientists who're actively utilizing the expertise of their laboratories. in response to their first-hand adventure the authors additionally offer important notes and troubleshooting sections.
Topics variety from usual suggestions, corresponding to pronuclear microinjection of DNA, to extra refined and smooth tools, comparable to the derivation and institution of embryonic stem (ES) cellphone strains, with outlined inhibitors in phone tradition medium. furthermore, comparable themes with relevance to the sphere are addressed, together with international web-based assets, criminal concerns, colony administration, cargo of mice and embryos, and the 3 R’s: refinement, relief and replacement.
Read Online or Download Advanced Protocols for Animal Transgenesis: An ISTT Manual PDF
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Extra info for Advanced Protocols for Animal Transgenesis: An ISTT Manual
Patent No. 7,524,677: Mammalian Cultured Inner Cell Mass Cell Culture “Mammalian Cultured Inner Cell Mass Cell Culture Using a G1 Cell As Nuclear Donor” is the title of Patent No. , dated April 28, 2009. The abstract indicates that the invention relates to a method of reconstituting an animal embryo. The method involves transferring a diploid nucleus into an oocyte which is arrested in the metaphase of the second meiotic division. The oocyte is not activated at the time of transfer, so that the donor nucleus remains exposed to the recipient cytoplasm for a period of time.
Also claimed is use of at least two nucleotide sequences each encoding a heterologous polypeptide that are introduced into the avian embryo, such as heavy and light chains of an antibody. One dependent claim specifies that the DNA molecule is not a eukaryotic viral vector. This may have been included to avoid 18 Canady potential validity issues relating to the use of such viral vectors. This would be an example of a limitation a patentee might prefer not to have to include in the broadest independent claim for fear of needlessly narrowing the claim scope.
S. gov). One can hire a professional search service to assist in identifying patents that may block a particular area of endeavor, or search the patent database oneself. The identification of relevant patents is but a starting point in the analysis of freedom to operate. One must then review the claims and the related file history. Retaining the services of a qualified professional is strongly recommended prior to the investment of significant sums in practicing a technology. If the desired activity is blocked by one or more patent holders, one can explore the feasibility of working around the patent claims, seeking appropriate licenses, or entering into a joint development agreement.